The Improved Database Of Chimeric Transcripts and RNA-Seq Data

ChiTaRS 5.0 is the latest and most comprehensive chimeric transcript repository, with 111 582 annotated entries from eight species, including 23 167 known human cancer breakpoints. The database includes unique information correlating chimeric breakpoints with 3D chromatin contact maps, generated from public datasets of chromosome conformation capture techniques (Hi–C). In this update, we have added curated information on druggable fusion targets matched with chimeric breakpoints, which are applicable to precision medicine in cancers. The introduction of a new section that lists chimeric RNAs in various cell-lines is another salient feature. Finally, using text-mining techniques, novel chimeras in Alzheimer's disease, schizophrenia, dyslexia and other diseases were collected in ChiTaRS. Thus, this improved version is an extensive catalogue of chimeras from multiple species. It extends our understanding of the evolution of chimeric transcripts in eukaryotes and contributes to the analysis of 3D genome conformational changes and the functional role of chimeras in the etiopathogenesis of cancers and other complex diseases.ChiTaRS 5.0是当前最新且最全面的嵌合转录本（chimeric transcript）数据库，共收录来自8个物种的111582条注释条目，其中包含23167条已知人类癌症断裂点。该数据库涵盖了将嵌合断裂点与3D染色质相互作用图谱相关联的独特信息，此类图谱源自公开的染色质构象捕获技术（Hi–C）数据集。在本次更新中，我们新增了经人工甄选整理的可药物融合靶点信息，该信息与嵌合断裂点相匹配，可应用于癌症精准医学领域。新增的用于罗列各类细胞系中嵌合RNA的板块，亦是本版本的另一显著亮点。此外，团队借助文本挖掘技术，在ChiTaRS数据库中收录了阿尔茨海默病、精神分裂症、阅读障碍及其他疾病中的新型嵌合转录本。综上，这款升级后的数据库是一份覆盖多物种的大规模嵌合转录本目录，它深化了我们对真核生物中嵌合转录本演化过程的认知，同时可为3D基因组构象变化分析，以及探究嵌合转录本在癌症与其他复杂疾病发病机制中的功能角色提供支撑。

搜集汇总

背景与挑战

背景概述

ChiTaRS-2.1是一个改进的嵌合转录本和RNA-seq数据库，收录了人类、小鼠、果蝇等多个物种的数万条嵌合转录本。该版本扩展了实验数据证据，包括新增了经实验验证的sense-antisense嵌合转录本类型，并收集了约1400个人类癌症断点及其表达水平数据。