Circadian regulation of lung repair and regeneration. Circadian regulation of lung repair and regeneration

To elucidate the mechanistic basis of the regenerative deficiency seen with clock disruption we undertook an unbiased approach with single-cell RNA sequencing (scRNA-seq) of epithelial and mesenchymal cells from Bmal1-/-, Bmal1creERT2/+, and their respective WT littermates Overall design: Lungs from embryonic Bmal1-/-, Bmal1+/+ controls, Bmal1creERT2/+, and Bmal1creERT2neg littermates were dissected, and single-cell preparation was obtained as described above. Single cell suspension was sorted as DARQ7-CD31-CD45- lung cells