Capturing the redox structure of cofactor proteins by combining X-ray diffraction and UV-vis and Raman spectroscopy

The overall aim of the project is deciphering structures and reaction mechanisms of different cofactor proteins. In situ studies using single-crystal UV-vis and Raman spectroscopy is inevitable to both prove and trap different redox states and to monitor the potential X-ray radiation damage of cofactor proteins. The project focus on both developing this type of combination setups, and to obtain the true redox structure of different cofactor enzymes.