The diversity of cellular systems involved in carbonate precipitation by Escherichia coli

Climate change is increasing the need to limit levels of anthropogenic CO2 released into the atmosphere. One approach being investigated is to generate products based on microbially induced carbonate precipitation (MICP), which can trap CO2 as CaCO3. We recently identified a novel MICP pathway in bacteria that is initiated by Ca2+ toxicity in cells, causing extracellular CO2 to be trapped as CO32- by Escherichia coli, although the yield of precipitated CaCO3 remained low (in the milligram range). In this work, we used the E. coli Keio gene knock-out library to identify 54 genes involved in MICP in E. coli, which could be broadly characterized into four groups: central metabolism, iron metabolism, cell architecture, and transport. The role of central metabolism appears to be crucial in maintaining alkaline conditions surrounding the cell that promote CaCO3 precipitation. The role of iron metabolism was less clear, although the results suggest that growth rate influences the initiation of..., , # Data from: The diversity of cellular systems involved in carbonate precipitation by Escherichia coli

Dataset DOI: [10.5061/dryad.dncjsxmbf](10.5061/dryad.dncjsxmbf)

## Description of the data and file structure

These are the raw data used to build Figures 2, 3, 4, and 5 in the publication: The diversity of cellular systems involved in carbonate precipitation by *Escherichia coli.*

### Fig._2_Raw_Data.xlsx

Part A. Readings of pH from B4m agar plates containing the indicated calcium salt. Readings from three replicate plates were averaged together for each strain. WT is the K-12 strain of *E. coli*. The knockouts are from the Keio collection of single gene knockouts in *E. coli*.

Part B. OD600 readings of B4m containing calcium-propionate media. Time indicates when samples were taken from flasks for OD600 measurements. WT is the K-12 strain of *E. coli*. The knockouts are from the Keio collection of single gene knockouts in *E. coli*.

Part C. pH readings of B4m containing calcium...,