Table_5.xlsx

Localized cutaneous leishmaniasis (LCL) is a chronic disease characterized by ulcerated skin lesion(s) and uncontrolled inflammation. The mechanisms underlying the pathogenesis of LCL are not completely understood, and little is known about posttranscriptional regulation during LCL. MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression and can be implicated in the pathogenesis of LCL. We investigated the involvement of miRNAs and their targets genes in human LCL using publicly available transcriptome data sets followed by ex vivo validation. Initial analysis highlighted that miRNA expression is altered during LCL, as patients clustered separately from controls. Joint analysis identified eight high confidence miRNAs that had altered expression (−1.5 ≤ fold change ≥ 1.5; p

局部化皮肤利什曼病（LCL）是一种以溃疡性皮肤病变和失控性炎症为特征的慢性疾病。LCL的发病机制尚不完全明了，关于LCL转录后调控的了解甚少。miRNA（微RNA）是一种非编码的小型RNA，能够调控基因表达，并可能参与LCL的发病过程。本研究利用公开的转录组数据集，结合体外验证，探讨了miRNA及其靶基因在人类LCL中的作用。初步分析显示，LCL患者的miRNA表达发生改变，患者组与对照组形成明显分离。联合分析鉴定出八个高置信度的miRNA，其表达发生显著变化（变化倍数介于-1.5至1.5之间；p值未给出）。