a. Introduced restriction sites are underlined. Amino acid spacer (GGGS) at the N-terminus of GFP is in bold. Primer sequences for constructing expressing plasmids and qPCR.
Sequences of primers and fluorescence hybridization probes. For the different TRBV forward primers, one common reverse primer and one common hybridization probe were used. * For TRBV5, 6 and 7, wobble
