RNA-seq analysis of mouse pluripotent stem cells derived cells
收藏NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE175563
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资源简介：
To detect the role of OP9 stromal cells in our optimized 3D self-assembling peptide induction system followed by the OP9 coculture system. First, we used RNA-seq to analyze mouse pluripotent stem cells derived total cells at day5 between the 3D+OP9 and 3D+0.1% group in our hematopoietic differentiation system. In addition, to further evaluate hematopoietic transcriptome differences between Lin-Sca-1+c-kit+CD201+ cells and Lin-Sca-1+c-kit+CD201- cells, we used RNA-seq to analyze mouse pluripotent stem cells derived Lin-Sca-1+c-kit+CD201+ and Lin-Sca-1+c-kit+CD201- cells at day5 in our 3D+OP9 hematopoietic differentiation system.Meanwhile,we used the mouse embryonic stem cell(mESC) as negative control and bone marrow derived Lin-Sca-1+c-kit+CD201+ and fetal liver derived Lin-Sca-1+c-kit+CD201+ as positive control. two groups together: 3 sample of 3D+OP9 and 3 sample of 3D+0.1%gel; five groups together : 3 sample of D5-LSKCD201+ 3 sample of D5-LSKCD201- 3 sample of mESC 3 sample of BM-LSKCD201+ 3 sample of FL-LSKCD201+
创建时间：
2021-06-28
