3' RACE coupled with Nanopore long-read sequencing

Nanopore long-read sequencing on 3' RACE products of the TAF1 locus (amplicons spanning TAF1 exon 30 to polyA) in XDP pre-frontal cortex samples (n=6), cerebral organoids (day 40) at physiological condition (n=9 for both XDP and controls) and treated with dBET6 (100 nM, 6 h; n=2 for both XDP and controls). Nanopore long-read sequencing on 3' RACE products of two distinct TAF1 reporters (CMV-driven and TAF1 promoter-driven) at physiological condition and DMSO or dBET6 (100nM, 18h) treated.