BM-2 (2018-5) Bone Marrow Microphysiological System within Chip Monitoring Test (Repeated Measures)

The purpose was to test within chip monitoring of cells within the BM MPS using extended AZ1 treatment and recovery. This tested if monitoring one chip through the duration of the study was possible and reduced variability, called repeated measures. This experiment also tested if repeated measures allow detection of toxicity at doses lower than 10uM AZ1 and allow confident detection of recovery.
Conclusion:
The repeated measures study design minimizes chip variability, and treatment effects can be detected by measuring cells from the media alone.
Notes:
In this repeated measures study design, the cells were sampled from the media well of the chip at each timepoint. In addition, the scaffold well was also sampled on the last timepoint at Day 28, where the samples are marked with an 'S'. Cells were also samples from the media well of the chip on Day 28, labelled as 'F' for floating. To standardize voltage settings across timepoints, single color controls were included each day to calculate compensation. Each dosed group had 6 replicates.