Triplexes are Dynamic DNA Secondary Structures Formed in vivo and Linked to Genome Instability [pacBio WGS]. Triplexes are Dynamic DNA Secondary Structures Formed in vivo and Linked to Genome Instability [pacBio WGS]

Numerous indirect, genetic approaches in various experimental systems have implicated dynamic alternative non-B DNA structures in human physiology and disease. Nevertheless, direct detection of dynamic DNA structures in vivo remains a challenge. To address this problem, we developed a new methods to visualize ssDNA containing alternative DNA structures. We described an uncharted landscape of dynamic alternative DNA structures formed by DNA cruciform and DNA triplexes (H-DNA) that contribute to genome instability. Overall design: Whole genome sequencing in APH pulsed cells to check mutagenesis at H-DNA repeats under replication stress.