Gene expression profile at single cell level of podocyte-specific I-PpoI expressing mice kidney cortex

To investigate the alterations in cell populations in the kidney after podocyte DNA DSBs, we performed single-cell RNA sequencing (scRNA-seq) on the renal cortex of 12-week-old podocyte-specific I-PpoI expressing mice that had not yet developed glomerulosclerosis or fibrosis. Kidneys were harvested, minced into 1 mm3 cubes, digested using digestion buffer and analyzed using scRNA-seq.