Enforced MAB21L4 expression enhances epidermal diferentiation. Enforced MAB21L4 expression enhances epidermal diferentiation

RNA-seq was used to define the role of MAB21L4 in epidermal differentiation by placing primary human keratinocytes transduced to overexpress MAB21L4 onto human dermis and grown for three days at the air-liquid interface to generate organotypic skin tissue. Overall design: Total RNA was then isolated from human skin organoids using QIAshredder (Qiagen) and Trizol (ThermoFisher) followed by DNA removal with the TURBO DNA-free kit (Ambion) according to the manufacturer’s instructions. RNA integrity was verified using an Agilent 2100 Bioanalyzer. RNA-Seq libraries were prepared with the mRNA Seq Sample Prep Kit (Illumina, Inc.) as recommended by the manufacturer. 100 bp paired-end sequencing reads were obtained using the Illumina HiSeq platform.