Additional file 2 of Extracellular vesicles from the apoplastic fungal wheat pathogen Zymoseptoria tritici

Additional file 2: Table S1. Metadata for EV samples/fungal cultures presented in Fig. 1 and Fig. 2. Table S2. List of proteins identified in ≥ 4/5 biological replicate samples of Z. tritici WAI332 EVs. Table S3. List of proteins identified in ≥ 2/3 SS samples, with at least 2 unique peptides detected using LC–MS/MS. Proteins found in the SS samples and also in EVs are indicated. Table S4. List of Z. tritici WAI332 EV proteins predicted to be effectors by EffectorP. Proteins were included when predicted as effectors by both EffectorP v1.0 and v2.0. Table S5a. List of studies used to source fungal EV proteins to define orthogroups with Orthofinder. Table S5b. Summary of proteins used for Orthofinder analysis. Table S6. OrthoFinder output showing fungal EV proteins grouped by orthogroup (OG) and species. Uniprot-KB or ensembl protein names are followed by a species ‘code’ defined for Orthofinder. CAAL = C. albicans; CRNE = C. neoformans, FOTG = F. oxysporum f. sp. vasinfectum; HICA = H. capsulatum; PABR = P. brasiliensis; SACE = S. cerevisiae; ZYTR = Z. tritici. Table S7. List of proteins identified with ≥ 2 unique peptides in ≥ 4/5 cell lysate (CL) samples (n = 533). Table S8. Potential Z. tritici WAI332 homologues for C. albicans EV marker proteins defined by Dawson et al., (2020). Homologous proteins were identified using reciprocal blastp searches of marker proteins against predicted proteins encoded in the Z. tritici WAI332 genome. Table S9. This data was generated by Orthofinder (v 2.3.11), using EV proteins described in previous fungal EV studies. Protein sequences were downloaded from Uniprot or Ensembl fungi and duplicate sequences removed within each species. Information on the studies listed and the proteins from each species is shown in Table S5.