Polarized B -cell functions. Polarized B -cell functions

In this study, we designed an in vitro approach to help us understand how extracellular components participate in human B cell functional plasticity and how this network might be involved in auto-immune diseases. Two activated T cell subsets were used to expose a common pool of B cells to distinct, controlled microenvironments. We showed that the same B cell population could experience two possible opposite evolutions, to either helper or suppressor functions, in a flexible manner. These cells were thus referred to as effector B cells (Eff B) or suppressor B cells (Supp B). We thus analyzed their different transcriptional programs by RNA sequencing. Overall design: B cells cultured with memory or naive T cells were analyzed by RNA sequencing