Using CRISPR/Cas9-assisted genome-editing tools, we constructed 68 efflux pump mutants of M. abscessus and investigated their roles in drug resistance and virulence. We found that different efflux pum
RNA-Seq on common bacterial pathogens in the presence vs absence of a variety of antibiotics. Two susceptible and two resistant isolates were exposed to antibiotics in laboratory culture, total RNA wa
Carbapenem-resistant Gram-negative bacteria are considered as a major threat to global health. Imipenem (IMP) is used as a last line of treatment against these pathogens but its efficiency is diminish
