Gene expression changes in neonatal and adult myeloid dendritic cells (mDC) upon TLR7/8 stimulation

We want to define the differences in gene expression changes between neonatal and adult myeloid dendritic cells upon stimulation with TLR7/8. These differences may point to specific signaling pathways which, in turn, may account for the known differential immune response of babies to vaccination or infection when compared to adults. Total RNA obtained from MACS-purified human mDC (using Miltenyi's CD1c+ Dendritic Cell Isolation Kit) stimulated with the synthetic TLR7/8 ligand 3M-003 (at final concentration of 5 uM) for 1 or 6 hours in vitro compared to unstimulated mDC.