Total RNA-seq using mouse tissues from Cyp2e1 +/+ mice upon pair-fed, Cyp2e1 +/+ mice upon EtOH-fed, Cyp2e1 -/- mice upon pair-fed, and Cyp2e1 -/- mice upon EtOH fed.

To investigate the effect of Cyp2e1 and EtOH on mRNA expression in mouse tissues, we profiled total RNA expression using mouse tissues from Cyp2e1 -/- mice and Cyp2e1 +/+ mice upon EtOH fed mice compared to control. We compare the expression level of RNA and profile circular RNA expression using circular RNA junction. Overall design: We prepared Cyp2e1 -/- and Cyp2e1 +/+ mice for making binge alcohol mouse model and control. After we prepared binge alcohol mouse model and control, brain, intestine, and liver tissues from the mice were isolated for total RNA-seq.