Effect of heat shock and competition on the antioxidant system of round goby

Monitoring oxidative stress biomarkers has become a powerful and common tool to estimate organismal condition and response to endogenous and environmental factors. In the present study, we used round goby (Neogobius melanostomus) from non-native European populations, as a model species to test sex differences in oxidative stress biomarkers. Considering sex differences in reproductive investment, we hypothesized that males would display lower resistance to abiotic stress. Fish were exposed to a heat shock (temperature elevated by 10°C) for 1h, 6h, and 12h and catalase activity (CAT), reduced glutathione (GSH), total antioxidant capacity (TAC) and lipid peroxidation (LPO) were measured in liver and muscle tissues. Liver of males was significantly more responsive compared to liver of females in all tested parameters. GSH was found to be the most responsive to heat stress exposure in both sexes. The results supported our hypothesis that male reproductive investment (territoriality, courtshi..., ,监测氧化应激生物标志物（oxidative stress biomarkers）已成为评估机体状态及生物体对内源性与环境因素应答反应的高效通用手段。本研究以欧洲非原生种群中的圆虎鱼（round goby，Neogobius melanostomus）作为模式物种，探究其氧化应激生物标志物的性别差异。鉴于繁殖投入存在性别差异，我们提出假说：雄性对非生物胁迫的抵抗力更弱。实验中将受试鱼类暴露于升高10℃的热休克环境中，分别处理1小时、6小时与12小时，随后检测其肝脏与肌肉组织中的过氧化氢酶活性（CAT）、还原型谷胱甘肽（GSH）、总抗氧化能力（TAC）及脂质过氧化（LPO）水平。结果显示，在所有检测参数中，雄性肝脏的应答反应显著强于雌性肝脏。还原型谷胱甘肽（GSH）是两种性别个体中对热应激应答最为敏感的指标。本研究结果支持我们的假说：雄性的繁殖投入（领域行为、求偶……）