Summary of library screening conditions, amino acid substitutions accumulated in PpAzoR variants and initial activity and thermostability relative to their parents.

1)Ratio of the initial activity of the variant to the parent type (Aiv/Aip), 2)Ratio of the residual activity to the initial activity of the variant (v), normalized to the parent type (p) – (Ar/Ai)v/(Ar/Ai)p The parents for the next generations are in bold.