Excessive Lipolysis and Inflammatory Response in Adipose Tissue Are Associated with Elevated Serum Growth Hormone in Dairy Cows with Clinical Ketosis

Supplemental Table 1. Basic description of the control and clinical ketotic cowsSupplemental Table 2. Ingredients and chemical compositions of the diets fed to dairy cows during the experimentSupplemental Table 3. The primer sequences (For, forward; Rev, reverse)Supplemental Table 4. Effects of different concentrationsof growth hormonetreatment (0, 5, 10 or 15 ng/mL) on glycerol content in supernatant and triglyceride content in bovine adipocytesSupplemental Table 5. Effects of different timesof growth hormonetreatment (0, 4, 8 or 12 h) on glycerol content in supernatant and triglyceride content in bovine adipocytesSupplemental Table 6. Effects of different concentrationsof growth hormonetreatment (0, 5, 10 or 15 ng/mL) onphosphorylation levels of IκBα and NF-κBand relative protein abundance of IκBα in bovine adipocytesSupplemental Table 7. Effects of different concentrationsof growth hormonetreatment (0, 5, 10 or 15 ng/mL) on mRNA abundance of TNFA, NLRP3, IL18, CASP1 and IL1B and the activity of caspase 1 in bovine adipocytesSupplemental Table 8. Effects of different timesof growth hormonetreatment (0, 4, 8 or 12 h) on phosphorylation levels of IκBα and NF-κBand relative protein abundance of IκBα in bovine adipocytesSupplemental Table 9. Effects of different timesof growth hormonetreatment (0, 4, 8 or 12 h) on mRNA abundance of TNFA, NLRP3, IL18, CASP1 and IL1B and the activity of caspase 1 in bovine adipocytes