five

Protointrons in Saccharomyces sp.

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE90105
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We sequenced non-ribosomal RNA from a upf1 deletion mutant of S. cerevisiae, and from wild type S. mikatae and S. bayanus, and identified a new class of yeast introns. Yeast non-ribosomal RNAs were isolated from vegetatively growing cells or cells treated with rapamycin for 60 minutes to mimick starvation, RNA was sequenced, and splice junctions were identified and validated.
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2024-04-17
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