Hyperspectral Imaging Dataset for Laser Thermal Ablation Monitoring in Vital Organs

Objectives: The objective of the research was to use hyperspectral imaging (HSI) to detect thermal damage induced in vital organs (such as the liver, pancreas, and stomach) during laser thermal therapy. The experimental study was conducted during thermal ablation procedures on live pigs.

Ethical Approval: The experiments were performed at the Institute for Image Guided Surgery in Strasbourg, France. This experimental study was approved by the local Ethical Committee on Animal Experimentation (ICOMETH No. 38.2015.01.069) and by the French Ministry of Higher Education and Research (protocol №APAFiS-19543-2019030112087889, approved on March 14, 2019). All animals were treated in accordance with the ARRIVE guidelines, the French legislation on the use and care of animals, and the guidelines of the Council of the European Union (2010/63/EU).

Description: During our experimental study, we used a TIVITA hyperspectral camera to acquire hypercubes of size 640x480x100 voxels, indicating 640x480 pixels for 100 bands, and regular RGB images at each acquisition step. These bands were acquired directly from the hyperspectral camera without additional pre-processing. The hypercube was acquired in approximately 6 seconds and synchronized with the absence of breathing motion using a protocol implemented for animal anesthesia. Polyurethane markers were placed around the target area to serve as references for superimposing the hyperspectral images, which were acquired using target areas selected according to the hyperspectral camera manufacturer's guidelines.

As part of our investigation, we included hyperspectral cubes from 20 experiments conducted under identical conditions in our study. The hyperspectral cubes were collected in three distinct stages. In the first stage, the cubes were gathered before laparotomy at a temperature of 37°C. In the second stage, we obtained the cubes as the temperature gradually increased from 60°C to 110°C at 10°C intervals. Finally, in the last stage, the cubes were collected after turning off the laser during the post-ablation phase. Thus, we obtained a total of 233 hyperspectral cubes, each consisting of 100 wavelengths, resulting in a dataset of 23,300 two-dimensional images. The temperature changes were recorded, and the “Temperature profile during laser ablation” image illustrates the corresponding profile, highlighting the specific time intervals during which the hyperspectral camera and laser were activated and deactivated. To provide a visual representation of the collected data, we have included several examples of images captured from different organs in the “Examples of ablation areas” figure.

The raw dataset, comprising 233 hyperspectral cubes of 100 wavelengths each, was transformed into 699 single-channel images using PCA and t-SNE decompositions. These images were then divided into training and test subsets and prepared in the COCO object detection format. This COCO dataset can be used for training and testing different neural networks.

Access to the Study: Further information about this study, including curated source code, dataset details, and trained models, can be accessed through the following repositories:

Source code: https://github.com/ViacheslavDanilov/hsi_analysis

Dataset: https://doi.org/10.5281/zenodo.10444212

Models: https://doi.org/10.5281/zenodo.10444269