Effect of intraperitoneal administration of minocycline on mechanical allodynia (Fig. 4B).

To assess mechanical allodynia, von Frey test was performed. The number of paw withdrawal times in response to a 0.166 g von Frey filament was counted and scored as followed; One trial involved 10 applications of filaments every 3 or 4 s, each of which was scored as 0, 1 or 2 as described in the methods section. The trial was evaluated based on a total score of 0–20 at the culmination. % of max score = (total score/2 10 trials) 100. Data show mean ± s.e.m. (n = 5–10).
*p<0.05,
**p<0.01 versus Sham-Vehicle,
##p<0.01 versus SNL-Vehicle.
d, days after SNL-operation; m, days of minocycline administration.