Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMAC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.70A resolution

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMAC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.70A resolution Descriptor: 16S Ribosomal RNA, 23S Ribosomal RNA, 30S ribosomal protein S10, ... Authors: Syroegin, E.A, Aleksandrova, E.V, Kruglov, A.A, Paranjpe, M.N, Svetlov, M.S, Polikanov, Y.S. Deposit date: 2025-04-07 Release date: 2025-06-25 Last modified: 2026-03-04 Method: X-RAY DIFFRACTION (2.7 Å) Cite: Structural insights into context-specific inhibition of bacterial translation by macrolides. Biorxiv, 2025

野生型嗜热栖热菌（Thermus thermophilus）70S核糖体与大环内酯类红霉素、信使RNA（mRNA）、氨酰化A位赖氨酰-tRNA^Lys、P位fMAC-肽酰-tRNA^Met以及脱酰基E位赖氨酰-tRNA^Lys形成复合物的晶体结构，分辨率达2.70埃。描述项：16S核糖体RNA（16S Ribosomal RNA）、23S核糖体RNA（23S Ribosomal RNA）、30S核糖体蛋白S10等……作者：Syroegin, E.A、Aleksandrova, E.V、Kruglov, A.A、Paranjpe, M.N、Svetlov, M.S、Polikanov, Y.S。提交日期：2025-04-07；发布日期：2025-06-25；最后修改日期：2026-03-04。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率2.7埃。引用文献：《大环内酯类化合物对细菌翻译的语境特异性抑制的结构解析》，BioRxiv，2025年