Bioinformatics Analysis of Q0D1P0 Gene of Mangrove Endophytic Fungus Aspergillus terreus

[Objectives] To analyze the gene structure of the protein that predicts 6-hydroxymellein synthase of Aspergillus terreus and predict the characteristics and functions of the protein structure encoded by the gene. [Methods] Various information analysis tools in NCBI, CBS and ExPASy websites were adopted. [Results] The Q0D1P0 gene had a full length of 2 954 bp, with 952 amino acids in the coding area, and Q0D1P0 had the highest homology with the hypothetical protein ATETN484_0003008800. The molecular weight of Q0D1P0 protein was 105 040.56, the theoretical isoelectric point (pI) was 5.69 and the grand average of hydropathieity was -0.242. It was speculated that Q0D1P0 was an unstable and non-secretory hydrophilic protein located in cytoplasm without transmembrane domain or signal peptide. It could be predicted that the secondary structure of Q0D1P0 encoding protein consisted mainly of random coil, α-helix and a PKS-DH anhydrase domain. [Conclusions] The results will lay a theoretical foundation for cloning and expression of 6-hydroxymellein synthase and further understanding of its activity and function.