Acropora aspera coral fecundity in reef slope and reef flat habitats in Heron Island (Southern Great Barrier Reef, Capricorn Bunker)

The dataset contains information of Acropora aspera coral fecundity in reef flat and reef slope habitats. All data originates from Heron Island corals in the southern Great Barrier Reef (Capricorn Bunker). \nIn November 2021, a total of 40x colonies of Acropora aspera were checked for presence of eggs, tagged, and sampled on the reef flat (100m), the sampling was proceeded in the following way:\n-\t10x colonies were tagged on the reef flat and 10x colonies were tagged on the reef slope \n-\t1x fragment was collected from each tagged colony and preserved in 5% formalin and seawater (total of 40x fragments)\nThe fixed samples were then decalcified in 3-10% hydrochloric acid. For each decalcified sample, 30 polyps were randomly selected and inspected for presence/absence of eggs to estimate percentages of reproductive polyps within a branch. Among the polyps inspected for each sample, a minimum of 10 gravid polyps were dissected under the microscope to identify, count, and measure ovaries, spermaries, and eggs. Scaled pictures of each polyp were taken with a camera for measurements. For size measurements, the width and length of each element are recorded. Measurements were performed with ImageJ.\n\nAccess:\nMetadata is fully public. Data files will be made fully public once the research has been published.\nFor special requests, please directly contact the Data Custodian and CC the Project Leader.\n\n\nLineage: NA – the dataset is the raw dataset prior to cleaning. It contains all necessary information to conduct cleaning by the user.

本数据集涵盖了粗枝鹿角珊瑚（Acropora aspera）在礁坪（reef flat）与礁坡（reef slope）两种生境下的繁殖力相关数据。所有数据均采集自大堡礁南部卡普里康-邦克群岛区（Capricorn Bunker）的赫伦岛（Heron Island）珊瑚种群。
2021年11月，研究人员对总计40株粗枝鹿角珊瑚开展卵存在性检测、标记与采样工作，其中礁坪区域采样范围为100米，具体采样流程如下：
- 在礁坪与礁坡区域各标记10株珊瑚
- 从每株标记珊瑚上采集1个组织碎片，使用5%福尔马林海水混合液固定（总计采集40份组织碎片）
固定后的样本随后在3%-10%的盐酸溶液中完成脱钙处理。针对每份脱钙后的样本，随机选取30个水螅体（polyp），检测其是否携带卵粒，以此估算单个分枝中具繁殖能力的水螅体占比。对于每份样本的检测水螅体，至少选取10个怀卵水螅体在显微镜下进行解剖，以识别、计数并测量卵巢（ovaries）、精巢（spermaries）与卵粒。使用相机拍摄每个水螅体的比例尺照片，用于后续尺寸测量。尺寸测量环节将记录各结构的长与宽，所有测量操作均通过ImageJ软件完成。

数据获取方式：
元数据已完全公开。研究论文正式发表后，完整数据文件将同步公开。
如有特殊需求，请直接联系数据管理员，并抄送项目负责人。

数据谱系：无（NA）——本数据集为清洗前的原始数据集，包含用户自主开展数据清洗所需的全部必要信息。