Essential genes whose expression is repressed by CTR9 depletion.

Genes were selected by the following criteria: (a) mRNA decreased more than 20-fold by CTR9 RNAi and (b) A significant decrease of at least 5-fold in representation in the RNAi library, using bloodstream forms either 3 or 6 days after tetracycline addition [48]. “rpkm” is the value for wild-type cells from RNASeq: an rpkm value of 20 is equivalent to one mRNA per cell [30]. “RNAi” is the rpkm for CTR9 RNAi divided by that for wild-type. “BS3” indicates the representation of this gene in RITSeq analysis in bloodstream forms, at day 3 after RNAi induction, divided by the representation without tetracycline; *not significant [48]. “BS6” is the same for 6 days of tetracycline.