High-throughput capture of actively transcribed region-interacting sequences reveals an intricate promoter-centered regulatory network

1. FigS10A_CTCF_VSCTCF_IAA_WB: Western blot analysis of CTCF before and after IAA-induced degradation in K562 cells. 2. FigS1E_dotplot: Dot blot assay for different incubation time points of Large (Klenow) Fragment and dNTPs (with biotin-dCTP).1. FigS10A_CTCF_VSCTCF_IAA_WB：针对K562细胞中吲哚-3-乙酸（IAA）诱导降解前后的CCCTC结合因子（CTCF）的蛋白质印迹法（Western blot，WB）分析。 2. FigS1E_dotplot：针对使用生物素标记脱氧胞苷三磷酸（biotin-dCTP）的克列诺大片段（Large (Klenow) Fragment）与脱氧核糖核苷三磷酸（dNTPs）的不同孵育时间点的斑点印迹法（Dot blot assay）检测。