Leaf transcriptome profiling of contrasting sugarcane genotypes for drought tolerance under field conditions. Leaf transcriptome profiling of contrasting sugarcane genotypes for drought tolerance under field conditions

Transcriptional profiling of sugarcane leaf (+1) was collected from drought-prone areas at 42 and 117 days after the last rainfall when plants were 6- and 9-month-old, respectively. Sample hybridization (non-irrigated treatment against irrigated control) was performed to monitor gene expression in a two-color Agilent custom microarray. Our goal was to investigate the effect of mild and severe water stress on the transcriptomes of drought-sensitive (IACSP97-7065) and -tolerant (IACSP94-2094) sugarcane genotypes. Overall design: Two-condition experiment: irrigated (control) vs. non-irrigated at 42nd day after the last rainfall (mild water stress), irrigated (control) vs non-irrigated at 117th day after the last rainfall (severe water stress).