Summary of CNS histological analyses.

aHistological assessment of classical EAE was assessed in 4 Lewis rats immunized with 50 µg GP69-88 in CFA. Histological assessment of atypical EAE was based on 10 Lewis rats immunized with 200 µg rat IgV-MOG in CFA and boosted with MOG-IgV/IFA on days 21 and 35. Rats were euthanized at peak disease. bRepresentative sections are shown in Figures 4–6. In rats with classical EAE, approximately 100% of the lesion area in the CNS consisted of punctate areas of mononuclear perivascular infiltration. In rats with atypical EAE, nearly 100% of the lesion area in the CNS was comprised of confluent lesions which were readily discernable by the unaided eye on H&E sections as a dark blue in contrast to the light purple of surrounding normal tissue. Confluent lesions typically involved areas greater than 1 mm2 and ranged from 1 mm to greater than 1 cm in one dimension, particularly in the spinal cord or at the base of the brainstem. Summary of CNS histological analyses.