Analysis of the Role of NuMA (TDIF) in DNA repair Through Next Generation Sequencing Techniques [NuMA (TDIF) CUT&RUN SH-SY5Y]

Purpose: To assess the role of NuMA (TDIF) in DNA repair Methods: CUT&RUN was carried out in untreated and hydrogen peroxide treated SH-SY5Y cells in triplicate. The data was mapped using bwa mem and subsequently used to generate bigwigs showing fold-change relative to inputs using MACS2 and various meta-feature profiles using the CGAT suite of bioinformatics tools Results: The data revealed increased occupancy of NuMA (TDIF) at promoters and enhancers, which increases upon hydrogen peroxide treatment Conclusions: Our study indicates that NuMA (TDIF) plays a role in the protection from and response to oxidative damage. This protective effect appears to be particularly strong at promoter and enhancer regions, and may differ between distinct subsets of gene categories. Overall design: Assessment of NuMA (TDIF) binding within the genome through NuMA (TDIF) CUT&RUN in SH-SY5Y cells under untreated and hydrogen peroxide treated conditions in triplicate. MH = Untreated/H2O2-, PH = H2O2 treated, IG = IgG T = NuMA (TDIF)