A nanovaccine for immune activation and prophylactic protection of atherosclerosis in mouse models

The raw flow cytometry files uploaded contain the activation of bone marrow derived dendritic cells (BMDCs), the proliferation profile after co-culture of BMDCs and T lymphcytes. We also analyzed splenic and lymphatic cells from C56BL/6J and ApoE knockout (KO) mice after vaccination of our nanovaccines (D+P, SP-D1P1, SP-D1+P1, and SP-P)., For in vitro experiments such as to check the surface expression of different markers, the collected cells were stained with antibodies before staining with Zombie Aqua fixable viability dye  Finally, the cells were resuspended in 200 µL FACS buffer (5 mM EDTA and 1% FBS in PBS) for flow cytometry analysis by BD FACSCantoII or a BD FACSCeleste cell analyzer. Data were analyzed by the FlowJo software (Tree Star) or the FCS Express 7 software (Reachsoft).
For in vivo experiments at 10 weeks and 26 weeks of age, the immunized ApoE-/- mice were anesthetized for blood collection and euthanized as indicated above. The spleens and dLNs were excised, minced, and filtered (70 μm; BD Biosciences) to generate a single-cell suspension and centrifuged at 4 °C at 1800 rpm for 10 min. After lysis of red blood cells, the pelleted splenocytes were resuspended in FACS buffer together with antibodies. The dLNs were extracted and passed through a 70 μm cell strainer, stained with antibodies. The cells..., , # Data from: A potent athero-protective nanovaccine cocktail by two-pronged intracellular delivery of antigen and adjuvant for dendritic cell activation

[https://doi.org/10.5061/dryad.zpc866tk7](https://doi.org/10.5061/dryad.zpc866tk7)

## Description of the data and file structure

The samples were named after the type of nanovaccines, antigens, and/or adjuvants administrated. The BMDCs were directly incubated with them while the animals were injected by subcutaneous injections at the ages of 7, 9, and 12 weeks.

Untreated mean negative control; PEG-SP indicates the superparamagnetic nanoparticles coated with PEG (nanoparticle control); p210 is the antigen used for managing atherosclerosis; CpG-ODN is the adjuvant. D+P indicates the physical mixture of CpG-ODN and p210; Sp-D indicates PEG-SP coated with only CpG-ODN; SP-P indicates PEG-SP coated with only p210; SP-DxPy indicates the PEG-SP co-delivering both CpG-ODN and p210, where x and y are the molar ratios of them; SP-Dx+Py indica...