A nanovaccine for immune activation and prophylactic protection of atherosclerosis in mouse models
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The raw flow cytometry files uploaded contain the activation of bone marrow derived dendritic cells (BMDCs), the proliferation profile after co-culture of BMDCs and T lymphcytes. We also analyzed splenic and lymphatic cells from C56BL/6J and ApoE knockout (KO) mice after vaccination of our nanovaccines (D+P, SP-D1P1, SP-D1+P1, and SP-P)., For in vitro experiments such as to check the surface expression of different markers, the collected cells were stained with antibodies before staining with Zombie Aqua fixable viability dye Finally, the cells were resuspended in 200 µL FACS buffer (5 mM EDTA and 1% FBS in PBS) for flow cytometry analysis by BD FACSCantoII or a BD FACSCeleste cell analyzer. Data were analyzed by the FlowJo software (Tree Star) or the FCS Express 7 software (Reachsoft). For in vivo experiments at 10 weeks and 26 weeks of age, the immunized ApoE-/- mice were anesthetized for blood collection and euthanized as indicated above. The spleens and dLNs were excised, minced, and filtered (70 μm; BD Biosciences) to generate a single-cell suspension and centrifuged at 4 °C at 1800 rpm for 10 min. After lysis of red blood cells, the pelleted splenocytes were resuspended in FACS buffer together with antibodies. The dLNs were extracted and passed through a 70 μm cell strainer, stained with antibodies. The cells..., , # Data from: A potent athero-protective nanovaccine cocktail by two-pronged intracellular delivery of antigen and adjuvant for dendritic cell activation [https://doi.org/10.5061/dryad.zpc866tk7](https://doi.org/10.5061/dryad.zpc866tk7) ## Description of the data and file structure The samples were named after the type of nanovaccines, antigens, and/or adjuvants administrated. The BMDCs were directly incubated with them while the animals were injected by subcutaneous injections at the ages of 7, 9, and 12 weeks. Untreated mean negative control; PEG-SP indicates the superparamagnetic nanoparticles coated with PEG (nanoparticle control); p210 is the antigen used for managing atherosclerosis; CpG-ODN is the adjuvant. D+P indicates the physical mixture of CpG-ODN and p210; Sp-D indicates PEG-SP coated with only CpG-ODN; SP-P indicates PEG-SP coated with only p210; SP-DxPy indicates the PEG-SP co-delivering both CpG-ODN and p210, where x and y are the molar ratios of them; SP-Dx+Py indica...
本数据集上传的原始流式细胞术（Flow Cytometry）文件包含骨髓源树突状细胞（bone marrow derived dendritic cells, BMDCs）的活化情况，以及BMDCs与T淋巴细胞共培养后的增殖谱。我们还分析了经本团队纳米疫苗（D+P、SP-D1P1、SP-D1+P1及SP-P）免疫后，C57BL/6J小鼠与载脂蛋白E敲除（ApoE knockout, ApoE KO）小鼠的脾脏细胞与淋巴结细胞。 针对检测不同标志物表面表达的体外实验，收集的细胞先经抗体染色，再用Zombie Aqua固定活性染料进行染色。最终将细胞重悬于200 μL FACS缓冲液（含5 mM EDTA与1%胎牛血清的磷酸盐缓冲液）中，使用BD FACSCantoII或BD FACSCeleste细胞分析仪进行流式细胞术分析。数据采用FlowJo软件（Tree Star公司）或FCS Express 7软件（Reachsoft公司）进行分析。 针对10周龄与26周龄的体内实验，对免疫后的ApoE-/-小鼠实施麻醉以采集血液，并按前述方法实施安乐死。剥离脾脏与引流淋巴结（draining lymph nodes, dLNs），剪碎后经70 μm滤膜（BD Biosciences）过滤以制备单细胞悬液，随后于4℃、1800 rpm离心10 min。红细胞裂解后，将沉淀的脾细胞重悬于FACS缓冲液中并加入抗体进行染色。引流淋巴结经提取后通过70 μm细胞筛过滤，再用抗体进行染色。相关细胞…… # 数据来源：通过双靶点细胞内递送抗原与佐剂激活树突状细胞的强效动脉粥样硬化保护性纳米疫苗组合 https://doi.org/10.5061/dryad.zpc866tk7 ## 数据与文件结构说明 样本根据所施用的纳米疫苗、抗原和/或佐剂类型命名。将骨髓源树突状细胞（BMDCs）直接与各试剂共孵育，而实验动物则于7、9、12周龄时接受皮下免疫接种。 未处理组为阴性对照；PEG-SP指经聚乙二醇（PEG）修饰的超顺磁性纳米颗粒（纳米颗粒对照）；p210为用于动脉粥样硬化防控的抗原；CpG-ODN为免疫佐剂。D+P指CpG-ODN与p210的物理混合物；Sp-D指仅包被CpG-ODN的PEG-SP纳米颗粒；SP-P指仅包被p210的PEG-SP纳米颗粒；SP-DxPy指同时共递送CpG-ODN与p210的PEG-SP纳米颗粒，其中x、y分别为二者的摩尔比；SP-Dx+Py indica……
