Cytosolic S100A8/A9 promotes Ca2+ supply at LFA-1 adhesion clusters during neutrophil recruitment

S100A8/A9 is an endogenous alarmin secreted by myeloid cells during many acute and chronic inflammatory disorders. Despite increasing evidence of the proinflammatory effects of extracellular S100A8/A9, little is known about its intracellular function. Here, we show that cytosolic S100A8/A9 is indispensable for neutrophil post-arrest modifications during outside-in signaling under flow conditions in vitro and neutrophil recruitment in vivo, independent of its extracellular functions. Mechanistically, genetic deletion of S100A9 in mice caused dysregulated Ca2+ signatures in activated neutrophils resulting in reduced Ca2+ availability at the formed LFA-1/F-actin clusters with defective β2 integrin outside-in signaling during post-arrest modifications. Consequently, we observed impaired cytoskeletal rearrangement, cell polarization and spreading, as well as cell protrusion formation in S100a9-/- compared to WT neutrophils, making S100a9-/- cells more susceptible to detach under flow, thereb..., , , # Data from: Cytosolic S100A8/A9 promotes Ca2+ supply at LFA-1 adhesion clusters during neutrophil recruitment

This dataset includes 8 different excel files, relevant to the manuscript: \"Cytosolic S100A8/A9 promotes Ca2+ supply at LFA-1 adhesion clusters during neutrophil recruitment\". [https://doi.org/10.5061/dryad.7d7wm384t](https://doi.org/10.5061/dryad.7d7wm384t)

5 excel files are the datasets of the 5 main figures and are named: FIGURE X_data availability.

3 excel files are the datasets of the 3 supplementary figures and are named: FIGURE X-FIGURE SUPPLEMENT X_data availability.

In each excel file there are different sheets, which contain the raw data of every single figure panel and are named after them (e.g. FIG 1A, FIG 3C...)

## Abbreviations

ICAM-1=Intercellular Adhesion Molecule 1

PSGL1=P-selectin glycoprotein ligand-1

LFA-1=lymphocyte function-associated antigen 1

Ca2+=Calcium ion

ER= endoplasmic reticulum

SOCE=store-operated calcium entry

## Descriptions

The Dat...