aThe amino acid mutant positions are in H5 numbering. bIFA were performed on 293T cells transfected with mutant rHA constructions. (+) to (+++) indicates the relative intensity of fluorescence.
aThe amino acid mutant positions are in H5 numbering.bIFA were performed on 293T cells transfected with mutant rHA constructions. (+) to (+++) indicates the relative intensity of fluorescence.
The goal of this study is to understand the effect of mutations to a seasonal H3 hemagglutinin using deep mutational scanning. We mutagenized the HA gene from the influenza strain A/Perth/16/2009(H3N2