Multi-organ quantitative proteome map following ASNase treatment

We applied the ultra-fast proteome profiling workflow to tissues collected from NSG mice treated with PBS (vehicle control), 20,000 U/kg ASNaseWT (Spectrila®), or 20,000 U/kg ASNaseQ59L for 0, 1, 3, 5, and 8 d. At each time point, mice were euthanized, and 13 tissues were collected, including subcutaneous white adipose tissue (SQWAT), gonadal adipose tissue (GAT), lung, spleen, stomach, small and large intestine, liver, kidney, heart, leg muscle, brain, and bone marrow. A total of 507 collected tissue samples were processed for quantitative proteome profiling.