Unraveling the effects of cold stratification and temperature on the seed germination of invasive Spartina alterniflora across latitude

Seed germination is critical to the life history of plants, playing an important role in the successful recruitment, colonization, and even invasion of new individuals within and outside population distribution ranges. Cold stratification and temperature are the key factors affecting seed germination traits. Studying how these two factors drive geographical variation in seed germination is essential to analyze and predict the geographical distribution range of alien plants in novel habitats. Spartina alterniflora, native to the United States, was introduced into China in 1979 and has spread over 20° of latitude along the eastern coast of China. Germination plays a crucial role in S. alterniflora’s large-scale invasion and diffusion across latitude. To evaluate the effects of cold stratification and temperature on seed germination of S. alterniflora across latitude, we collected seeds at seven locations across latitude in China. We exposed these provenances to cold stratification at 4°C (0, 1,...