Estimated V_mem values during OS and AD differentiation.

Vmem values for week 0 were taken from intracellular recordings of cells during early differentiation (Days 1–4) (Fig. 2A). Subsequent Vmem values were estimated from the DiSBAC fluorescence data (Fig. 1B): since bis-oxonol dyes such as DiSBAC typically generate a ∼1% change in fluorescence per 1 mV change [29], changes in Vmem for weeks 1–4 were estimated by calculating the percentage changes in fluorescence compared to week 0.