Correlation between phenotypic (LPT) and genotypic (sequencing) amitraz resistance status.

Discriminating dose of amitraz used was 250 ppm. a Larvae that were alive after the larval packet test assay was completed, meaning they were resistant to the amitraz concentrations applied. b Larvae that were susceptible to the concentrations of amitraz used and were dead after the assay was completed. c RF is the resistance factor, a RF of 100 implies that the strain is amitraz resistant. RF of 10 and 28 means the strain is susceptible to amitraz. d Genotype was inferred based on whether or not the sequenced larvae contained the two SNPs published by Chen, He (26). CC/CC – all four alleles are resistant (homozygous resistant), AA/TT – all four alleles are the wild type alleles (homozygous susceptible), AC/TC – at both SNP positions both the wild type and mutant alleles are present (heterozygous). Correlation between phenotypic (LPT) and genotypic (sequencing) amitraz resistance status.