Single-cell RNAseq of the mouse lung
收藏Alliance of Genome Resources2026-08-01 收录
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To define the hedgehog-interacting protein (Hhip) expression domain in adult mouse lung, we conduct single-cell RNAseq of the mouse lung using 10X scRNAseq technique. Adult mouse lung epithelial and mesenchymal cells were sorted based on EpCAM+ and EpCAM-/CD45-/CD31-, respectively. Dead cells were excluded using DAPI negative selection. The sequencing sample were mixed with half epithelial cells and half mesenchymal cells.
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