Expression data of mesenchymal stromal cells after 40 hours of co-culture with primary B-cell precursor acute lymphoblastic leukemia cells

B-cell precursor acute lymphoblastic leukemia (BCP-ALL) cells reside in the bone marrow microenvironment, where they are protected against chemotherapeutic agents. Mesenchymal stromal cells (MSCs) are key components of this supporting framework. The present study aimed to unravel whether MSCs derived from pediatric BCP-ALL patients (leukemic MSCs) differ from MSCs derived from healthy pediatric donors (control-MSCs). Therefore, we studied their gene expression profiles after 40 hours of co-culture with primary B-cell precursor acute lymphoblastic leukemia cells. MSCs were sorted using fluorescence-activated cell sorting (FACS). Six different primary mesenchymal stromal cells (MSCs) were cultured for 40 hours in the presence of primary B-cell precursor acute lymphoblastic leukemia cells (derived from 4 different pediatric BCP-ALL patients). Mesenchymal stromal cells were sorted from B-cell precursor acute lymphoblastic leukemia cells using a fluorescence-activated cell sorter (FACS). Microarray analyses were performed on these sorted MSCs. Limma analyses were performed to identify differentially expressed probesets in MSCs upon co-culture with BCP-ALL cells compared to mono-cultures of these MSCs. One sample was lost due to poor RNA quality.