Hippocampal GR and pCREB binding analysis of glucocorticoid enhanced memory formation [ChIP-seq]

Methods: Adult male rats received 3 minutes of object exploration training or no training either with a 3.0mg.kg post-training corticosterone injection or vehicle injection to study the molecular effects underlying enhanced memory formation. Hippocampi were dissected 45 minutes after injection and snap-frozen. Subsequently, Chromatin ImmunoPrecipitation was performed for GR and pCREB and the ChIP samples were sequenced.实验方法：成年雄性大鼠接受3分钟物体探索训练或不接受训练，同时于训练后注射3.0mg/kg皮质酮或溶剂对照，以研究记忆形成增强的潜在分子机制。于注射后45分钟剥离大鼠海马组织并快速冷冻保存。随后，针对糖皮质激素受体（GR）与磷酸化环腺苷酸应答元件结合蛋白（pCREB）开展染色质免疫沉淀（Chromatin ImmunoPrecipitation，ChIP）实验，并对ChIP样本进行测序。