Germination of pollen from <i>eIF3e⁺; FTL1413/eif3e</i>⁻<i>; +</i> heterozygous parents.

These plants did not produce tetrads (QRT1+). Among the germinated pollen tubes, a clear bias towards FL+ is evident (only 28% were FL-). The deviation from a 1∶1 segregation was significant at P<0.05 for each plant (Chi-square test). The ‘Total’ ratios did not deviate from 1∶1 (P>0.05). Based on the map distance between eIF3e and FTL1413 (7.7 cM) only about 8% of FL- pollen grains are expected to be eIF3e+. Therefore, it appears that eif3e− mutant pollen grains did germinate at a low percentage.