Gene identification.

We identified the corresponding GBT-tagged locus for each ZIP line. Consistent with the random nature of RP2 GBT insertion, no two insertions were on the same linkage group (LG). All eleven ZIP loci have mammalian homologs. Ten have human homologs, and one (msxcmn0245Gt) has a murine homolog but no reported human homolog. Mutations in two of those ten human homologs, FRAS1 and GRIP1, are known to be involved in Fraser Syndrome, a human disease whose phenotype includes blistering. Three ZIP alleles are novel integument genes: arhgef25bmn0175Gt, fkbp10bmn0316Gt, and megf6amn0325Gt. Although several ZIP alleles (§) were reported in an earlier publication, this study initially identified their expression patterns and includes previously unpublished data for each.§ Sequence identified in Clark et al., 2011 [17].‡ Novel integument genes.* GBT homozygous mutant phenocopies published ENU mutant at the designated locus.d Human homolog is a disease-related gene.Gene identification.