Paneth cell gene expression profiles after zinc suplementation in TNF context

Mice were given zinc as a salt (ZnSO4; 25 mM) in the drinking water for 7 days, then mice were injected with TNF and sampling was done 3 hours after injection. Expression profiling with RNA-seq was performed on purified paneth cellq to investigate the genome wide effects of zinc supplemenation on the paneth cell gene expression profile in the conext of TNF 2 groups of 4 mice each were given zinc in ultrapure water or just ultrapure water for 7 days. Afterard the were injected with TNF and paneth cells were isolated for sequencing 3 hours later