Extended investigation of tube-gel sample preparation: a versatile and simple choice for high throughput quantitative proteomics

We investigated the compatibility of tube-gel with alternatives to SDS-based buffers allowing notably the extraction of proteins in various pH conditions. We also explored the use of photopolymerization to extend the number of possibilities, as it is compatible with a wide range of pH and is non-oxydative. To achieve this goal, we compared six extraction buffers in combination with two polymerization conditions to further optimize tube-gel protocol and evaluate its versatility.