rDNA sequences of yeast, fungi, and bacteria isolated from Polish honey and bee bread samples

DNA fragments encoding D1/D2 domain of large subunit ribosomal RNA of yeast and fungi were PCR amplified using NL1 5’ GCATATCAATAAGCGGAGGAAAAG 3’ and NL4 5’ GGTCCGTGTTTCAAGACGG 3’ primers and sequenced by Sanger method. DNA encoding ITS1-5.8S-ITS2 fragments of ribosomal RNA (internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2) of yeast and fungi were PCR amplified using ITS1 5' TCCGTAGGTGAACCTGCGG 3' and ITS4 5’ TCCTCCGCTTATTGATATGC 3’ primers and sequenced by Sanger method. PCR amplification of bacterial 16S rDNA fragments was performed using fD1 5’ ccgaattcgtcgacaacAGAGTTTGATCCTGGCTCAG 3’ and rP2 5’ cccgggatccaagcttACGGCTACCTTGTTACGACTT 3’ primers and sequenced by Sanger method.