Data underlying the research on particle manipulation using hydrodynamic forcing, using a-priori particle manipulation algorithm and without Proportional-Integration-Derivative (PID) control.

The reseach objective is to present a microfluidic approach to achieve the dynamic control of particle pathlines within a flow through microfluidic device. Our approach combines the design of a flow-through microfluidic flow cell with the ability to manipulate the streamlines of the flow and an optimization procedure to find a priori optimal particle path-lines. The experimental raw images were recorded with a sCMOS camera (PCO) with a pixel pitch of 6.5 μm. The camera was mounted on a microscope (Nikon Eclipse Ti) with a 1x objective. The acquisition frequency was 5 Hz corresponding to an average in-plane displacement of 4-6 pixels between two consecutive recordings. The zip file contains the raw images and the MATLAB script of the following experiments by using hydrodynamic forcing only: 1. Single particle deflection upward 2. Single particle deflection downward 3. Single particle trap 4. Two particles separation 5. Two particles coming closer to eachother 6. Two particles interchanging their position