64 primer pairs developed and tested in silico for Salminus brasiliensis

The optimization 137 temperature was evaluated, and it was possible to select even more robust primers, yielding 64 primers pairs this value was obtained manually by the OligoAnalyser website that evaluates the presence of harpins and dimers of each primer.本研究对优化后的137℃反应温度进行了评估，同时可筛选出鲁棒性更强的引物（primer），最终获得64对引物（primer pair）。该筛选结果由OligoAnalyser网站手动计算得出，该工具可检测每条引物的发夹结构（hairpin）与二聚体（dimer）的存在情况。