Respiratory supercomplexes provide metabolic efficiency in zebrafish

The role of OXPHOS respiratory supercomplexes remains still unknown. To answer this question we use as a model a SCAF1 null allele model in zebrafish. SCAF1 (cox7a2l) is the only supercomplex assembly factor identified in vertebrates and it is involved in the physical link of III-IV complexes. Therefore the SCAF1 null allele model lack all III-IV interactions. We demonstrate that supercomplexes provide an advantage in the optimization of metabolic resources. Muscle from females SCAF1Δ1 after the 6 weeks of a regime of standard diet or double diet were dissected and stored at -80°C.